stemspan serum free expansion medium (sfem) (09650) (STEMCELL Technologies Inc)
90
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STEMCELL Technologies Inc
stemspan serum free expansion medium (sfem) (09650)
Stemspan Serum Free Expansion Medium (Sfem) (09650), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan+serum-free+expansion+medium+(sfem)+09650/stemspan+serum+free+expansion+medium/pmc11960508-207-5-12
Average 90 stars, based on 1 article reviews
Stemspan Serum Free Expansion Medium (Sfem) (09650), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan+serum-free+expansion+medium+(sfem)+09650/stemspan+serum+free+expansion+medium/pmc11960508-207-5-12
Average 90 stars, based on 1 article reviews
stemspan serum free expansion medium (sfem) (09650) - by Bioz Stars,
2026-09
90/100 stars
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Purification:Article Title: Neutrophils drive vascular occlusion, tumour necrosis and metastasis. Article Snippet: These HSPCs were grown in SFEM medium (StemSpan Serum-Free Expansion Medium, 09650, StemCell Technologies), 10% FBS, 20 ng ml−1 Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion. Article Snippet: Hematopoietic stem cell (HSCs) transplantation is the primary therapeutic modality used to treat hematopoietic disorders.. It centers on the capability of a small quantity of HSCs to repopulate whole blood lineages.. Along with limited availability of suitable donors, the need for sufficient number of donor HSCs is still challenging in clinical relevance. Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL Article Title: Macrophage depletion damages hematopoiesis partially through inhibition of cell homing and expansion after hematopoietic cell transplantation. Article Snippet: Bone marrow macrophages (Mφ) are essential components of the bone marrow niche that regulate the function of hematopoietic stem cells.. Poor graft function and inhibition of hematopoietic production can result from abnormal macrophage function; however, the underlying mechanism is unclear.. Clodronate liposomes (Clo-Lip) have been used widely to deplete macrophages and study their functions. Article Title: Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations Article Snippet: Cells were first prestimulated in StemSpan serum free expansion medium (SFEM) (09650, Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of Article Title: STAT1 inhibition promotes oxidative stress to sustain leukemia stem cell maintenance. Article Snippet: New strategy to prevent relapse and drug resistance in acute myeloid leukemia (AML) is urgently to be solved.. The connection between those properties and leukemia stem cells (LSCs) in AML remains poorly understood.. In this study, we demonstrate that leukemia cells with high signal transducer and activator of transcription 1 (STAT1) expression preserve quiescent properties, in contrast, leukemia cells with low STAT1 expression possess active and vulnerable apoptotic properties in AML model, highlighting the differential impact of STAT1 expression on cellular behavior in acute myeloid leukemia. Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion Article Snippet: SCF medium is defined as Cell Culture:Article Title: Neutrophils drive vascular occlusion, tumour necrosis and metastasis. Article Snippet: These HSPCs were grown in SFEM medium (StemSpan Serum-Free Expansion Medium, 09650, StemCell Technologies), 10% FBS, 20 ng ml−1 Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion. Article Snippet: Hematopoietic stem cell (HSCs) transplantation is the primary therapeutic modality used to treat hematopoietic disorders.. It centers on the capability of a small quantity of HSCs to repopulate whole blood lineages.. Along with limited availability of suitable donors, the need for sufficient number of donor HSCs is still challenging in clinical relevance. Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL Article Title: Macrophage depletion damages hematopoiesis partially through inhibition of cell homing and expansion after hematopoietic cell transplantation. Article Snippet: Bone marrow macrophages (Mφ) are essential components of the bone marrow niche that regulate the function of hematopoietic stem cells.. Poor graft function and inhibition of hematopoietic production can result from abnormal macrophage function; however, the underlying mechanism is unclear.. Clodronate liposomes (Clo-Lip) have been used widely to deplete macrophages and study their functions. Article Title: Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations Article Snippet: Cells were first prestimulated in StemSpan serum free expansion medium (SFEM) (09650, Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of Article Title: STAT1 inhibition promotes oxidative stress to sustain leukemia stem cell maintenance. Article Snippet: New strategy to prevent relapse and drug resistance in acute myeloid leukemia (AML) is urgently to be solved.. The connection between those properties and leukemia stem cells (LSCs) in AML remains poorly understood.. In this study, we demonstrate that leukemia cells with high signal transducer and activator of transcription 1 (STAT1) expression preserve quiescent properties, in contrast, leukemia cells with low STAT1 expression possess active and vulnerable apoptotic properties in AML model, highlighting the differential impact of STAT1 expression on cellular behavior in acute myeloid leukemia. Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion Article Snippet: SCF medium is defined as Recombinant:Article Title: Neutrophils drive vascular occlusion, tumour necrosis and metastasis. Article Snippet: These HSPCs were grown in SFEM medium (StemSpan Serum-Free Expansion Medium, 09650, StemCell Technologies), 10% FBS, 20 ng ml−1 Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion. Article Snippet: Hematopoietic stem cell (HSCs) transplantation is the primary therapeutic modality used to treat hematopoietic disorders.. It centers on the capability of a small quantity of HSCs to repopulate whole blood lineages.. Along with limited availability of suitable donors, the need for sufficient number of donor HSCs is still challenging in clinical relevance. Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL Article Title: Macrophage depletion damages hematopoiesis partially through inhibition of cell homing and expansion after hematopoietic cell transplantation. Article Snippet: Bone marrow macrophages (Mφ) are essential components of the bone marrow niche that regulate the function of hematopoietic stem cells.. Poor graft function and inhibition of hematopoietic production can result from abnormal macrophage function; however, the underlying mechanism is unclear.. Clodronate liposomes (Clo-Lip) have been used widely to deplete macrophages and study their functions. Article Title: Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations Article Snippet: Cells were first prestimulated in StemSpan serum free expansion medium (SFEM) (09650, Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of Article Title: STAT1 inhibition promotes oxidative stress to sustain leukemia stem cell maintenance. Article Snippet: New strategy to prevent relapse and drug resistance in acute myeloid leukemia (AML) is urgently to be solved.. The connection between those properties and leukemia stem cells (LSCs) in AML remains poorly understood.. In this study, we demonstrate that leukemia cells with high signal transducer and activator of transcription 1 (STAT1) expression preserve quiescent properties, in contrast, leukemia cells with low STAT1 expression possess active and vulnerable apoptotic properties in AML model, highlighting the differential impact of STAT1 expression on cellular behavior in acute myeloid leukemia. Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion Article Snippet: SCF medium is defined as Incubation:Article Title: Neutrophils drive vascular occlusion, tumour necrosis and metastasis. Article Snippet: These HSPCs were grown in SFEM medium (StemSpan Serum-Free Expansion Medium, 09650, StemCell Technologies), 10% FBS, 20 ng ml−1 Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion. Article Snippet: Hematopoietic stem cell (HSCs) transplantation is the primary therapeutic modality used to treat hematopoietic disorders.. It centers on the capability of a small quantity of HSCs to repopulate whole blood lineages.. Along with limited availability of suitable donors, the need for sufficient number of donor HSCs is still challenging in clinical relevance. Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL Article Title: Macrophage depletion damages hematopoiesis partially through inhibition of cell homing and expansion after hematopoietic cell transplantation. Article Snippet: Bone marrow macrophages (Mφ) are essential components of the bone marrow niche that regulate the function of hematopoietic stem cells.. Poor graft function and inhibition of hematopoietic production can result from abnormal macrophage function; however, the underlying mechanism is unclear.. Clodronate liposomes (Clo-Lip) have been used widely to deplete macrophages and study their functions. Article Title: Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations Article Snippet: Cells were first prestimulated in StemSpan serum free expansion medium (SFEM) (09650, Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of Article Title: STAT1 inhibition promotes oxidative stress to sustain leukemia stem cell maintenance. Article Snippet: New strategy to prevent relapse and drug resistance in acute myeloid leukemia (AML) is urgently to be solved.. The connection between those properties and leukemia stem cells (LSCs) in AML remains poorly understood.. In this study, we demonstrate that leukemia cells with high signal transducer and activator of transcription 1 (STAT1) expression preserve quiescent properties, in contrast, leukemia cells with low STAT1 expression possess active and vulnerable apoptotic properties in AML model, highlighting the differential impact of STAT1 expression on cellular behavior in acute myeloid leukemia. Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion Article Snippet: SCF medium is defined as Isolation:Article Title: Neutrophils drive vascular occlusion, tumour necrosis and metastasis. Article Snippet: These HSPCs were grown in SFEM medium (StemSpan Serum-Free Expansion Medium, 09650, StemCell Technologies), 10% FBS, 20 ng ml−1 Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion. Article Snippet: Hematopoietic stem cell (HSCs) transplantation is the primary therapeutic modality used to treat hematopoietic disorders.. It centers on the capability of a small quantity of HSCs to repopulate whole blood lineages.. Along with limited availability of suitable donors, the need for sufficient number of donor HSCs is still challenging in clinical relevance. Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL Article Title: Macrophage depletion damages hematopoiesis partially through inhibition of cell homing and expansion after hematopoietic cell transplantation. Article Snippet: Bone marrow macrophages (Mφ) are essential components of the bone marrow niche that regulate the function of hematopoietic stem cells.. Poor graft function and inhibition of hematopoietic production can result from abnormal macrophage function; however, the underlying mechanism is unclear.. Clodronate liposomes (Clo-Lip) have been used widely to deplete macrophages and study their functions. Article Title: Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations Article Snippet: Cells were first prestimulated in StemSpan serum free expansion medium (SFEM) (09650, Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of Article Title: STAT1 inhibition promotes oxidative stress to sustain leukemia stem cell maintenance. Article Snippet: New strategy to prevent relapse and drug resistance in acute myeloid leukemia (AML) is urgently to be solved.. The connection between those properties and leukemia stem cells (LSCs) in AML remains poorly understood.. In this study, we demonstrate that leukemia cells with high signal transducer and activator of transcription 1 (STAT1) expression preserve quiescent properties, in contrast, leukemia cells with low STAT1 expression possess active and vulnerable apoptotic properties in AML model, highlighting the differential impact of STAT1 expression on cellular behavior in acute myeloid leukemia. Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion Article Snippet: SCF medium is defined as FACS:Article Title: Neutrophils drive vascular occlusion, tumour necrosis and metastasis. Article Snippet: These HSPCs were grown in SFEM medium (StemSpan Serum-Free Expansion Medium, 09650, StemCell Technologies), 10% FBS, 20 ng ml−1 Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion. Article Snippet: Hematopoietic stem cell (HSCs) transplantation is the primary therapeutic modality used to treat hematopoietic disorders.. It centers on the capability of a small quantity of HSCs to repopulate whole blood lineages.. Along with limited availability of suitable donors, the need for sufficient number of donor HSCs is still challenging in clinical relevance. Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL Article Title: Macrophage depletion damages hematopoiesis partially through inhibition of cell homing and expansion after hematopoietic cell transplantation. Article Snippet: Bone marrow macrophages (Mφ) are essential components of the bone marrow niche that regulate the function of hematopoietic stem cells.. Poor graft function and inhibition of hematopoietic production can result from abnormal macrophage function; however, the underlying mechanism is unclear.. Clodronate liposomes (Clo-Lip) have been used widely to deplete macrophages and study their functions. Article Title: Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations Article Snippet: Cells were first prestimulated in StemSpan serum free expansion medium (SFEM) (09650, Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of Article Title: STAT1 inhibition promotes oxidative stress to sustain leukemia stem cell maintenance. Article Snippet: New strategy to prevent relapse and drug resistance in acute myeloid leukemia (AML) is urgently to be solved.. The connection between those properties and leukemia stem cells (LSCs) in AML remains poorly understood.. In this study, we demonstrate that leukemia cells with high signal transducer and activator of transcription 1 (STAT1) expression preserve quiescent properties, in contrast, leukemia cells with low STAT1 expression possess active and vulnerable apoptotic properties in AML model, highlighting the differential impact of STAT1 expression on cellular behavior in acute myeloid leukemia. Article Title: Development of a novel and synthetic HematoMiR technology that broadly modulates quiescence of stem cells and enhances HSC expansion Article Snippet: SCF medium is defined as |